Research Paper Volume 11, Issue 6 pp 1695—1715

Long non-coding RNA CDKN2B-AS1 reduces inflammatory response and promotes cholesterol efflux in atherosclerosis by inhibiting ADAM10 expression

Figure 6. Overexpression of CDKN2B-AS1 methylates ADAM10, inhibits inflammatory response, and promotes cholesterol efflux in atherosclerosis. (A) Oil red O staining of aortic was used to detect aortic plaque formation in C57BL/6J and ApoE-/- mice (× 10), the red arrows indicate the formed atheromatous plaque; (B) HE staining was used to detect arterial plaque formation in C57BL/6J and ApoE-/- mice (× 400), the red arrows indicate atheromatous plaque accompanied by foam cells after HE staining; (C) Liquid scintillation counter was applied to measure the effect of various intervention factors on serum cholesterol efflux in ApoE-/- mice; (D) Oil red O staining of aortic detection of intervention factors of aortic plaque formation on ApoE-/- mice (× 10), the arrows indicate the formed atheromatous plaque; (E) HE staining of aortic roots for detection of aortic plaque formation in ApoE-/- mice (× 400). (F) ELISA was used to detect the serum levels of IL-1β and TNF-ɑ in ApoE-/- mice; * p < 0.05 the M-oe-ADAM10 group versus the M-oe-NC group; # p < 0.05 the M-oe-CDKN2B-AS1 group versus the M-oe-NC group; the measurement data were expressed in the form of mean ± standard deviation and analyzed by unpaired t-test, n = 12; the experiment was repeated 3 times; HE, hematoxylin-eosin; ELISA, enzyme linked immunosorbent assay; TNF, tumor necrosis factor; IL, interleukin; CDKN, cell-dependent kinase inhibitor; ADAM10, A disintegrin and metalloprotease 10.