Figure 3. Aging effect on the number of macrophages in cardiac tissue. Representative images of cardiac cryosections stained with antibodies against (A-D) CD68, followed by (E) statistical analysis of CD68-positive cells per high power field (hpf). (F-K) CD206, followed by (L) statistical analysis of CD206-positive cells per high power field (hpf). The analyses were performed with myocard from young and old men (m) or women (w). Data are shown as the mean ± SEM (n= 6-8/group). Arrows show CD68- or CD206-positive cells.