Research Paper Volume 11, Issue 9 pp 2653—2669

Prostate enlargement and altered urinary function are part of the aging process

Figure 6. Aging leads to changes in collagen deposition. (A) Representative image of picrosirius red (PSR) staining of prostatic urethra specifically around the urethral lumen show predominantly green and yellow collagen fibers in young mice. (B) Representative image of PSR staining show predominantly orange and green collagen fibers in aged mice. (C) Quantitation of collagen fibers in the prostatic urethra show a statistical increase of orange and red collagen bundles in aged mice. (D) Representative image of PSR staining show predominantly green collagen fibers within the AP of young mice. (E) Representative image of PSR staining show predominantly yellow, orange, and red collagen bundles within the AP of aged mice. (F) Quantitation of collagen fibers within the AP shows a statistical increase in yellow, orange, and red collagen bundles and an overall increase in total collagen in aged mice. (G) Representative image of PSR staining show predominantly green collagen fibers within the VP of young mice. (H) Representative image of PSR staining show predominantly yellow and orange collagen bundles within the VP of aged mice. (I) Quantitation of collagen fibers within the VP shows a statistical increase in yellow and orange collagen bundles and an overall increase in total collagen in aged mice. (J) Representative image of PSR staining show predominantly green collagen fibers within the DLP of young mice. (K) Representative image of PSR staining show predominantly yellow and orange collagen bundles within the DLP of aged mice. (L) Quantitation of collagen fibers within the DLP shows a statistical increase in yellow and orange red collagen bundles and an overall increase in total collagen in aged mice. *, p-value < 0.05; **, p-value < 0.01