Research Paper Volume 11, Issue 9 pp 2670—2680

Downregulation of miR-125b promotes resistance of glioma cells to TRAIL through overexpression of Tafazzin which is a mitochondrial protein

Figure 5. MiR-125b mimics targets TAZ to enhance the TRAIL-dependent mitochondrial apoptosis in U251/R and U87/R. (A) MiR-125b mimics failed to promote the caspase-8 activation in TRAIL-treated U251/R and U87/R cells. (B) MiR-125b mimics targeted TAZ to the enhance the TRAIL-induced damage of mitochondrial membrane potential (MMP) of U251/R and U87/R. (C) Protein level of cytochrome c in mitochondria or cytoplasm faction of U251/R and U87/R cells. (D) MiR-125b mimics targeted TAZ to the enhance the TRAIL-induced activation of caspase-9 and -3 in U251/R and U87/R cells. (E) MiR-125b mimics targeted TAZ to enhance the TRAIL-dependent cell apoptosis of U251/R and U87/R. *P<0.05 vs. TRAIL + NCO group. #P<0.05 vs. TRAIL + miR-125b group.