Research Paper Volume 11, Issue 9 pp 2681—2698

Magnesium transporter protein solute carrier family 41 member 1 suppresses human pancreatic ductal adenocarcinoma through magnesium-dependent Akt/mTOR inhibition and bax-associated mitochondrial apoptosis

Figure 4. Bax-associated mitochondrial apoptosis is involved in the tumour-suppressive effects of SLC41A1. (A) Overexpression of SLC41A1 reduced the MMP, as indicated by JC-1 staining. (B) SLC41A1 activated Bax transcription and suppressed Bcl-2 transcription. (C) SLC41A1 increased Bax protein expression, cytochrome c release and cleavage of caspase-3, and reduced Bcl-2 protein level in PDAC cells. (D) Treatment of cells with a caspase inhibitor abrogated the tumour-suppressive effects of SLC41A1. (E) RNA interference of Bax abolished the tumour-suppressive effects of SLC41A1. *p < 0.05; **p < 0.01; ***p < 0.001.