Research Paper Volume 11, Issue 9 pp 2699—2723

Laminin α4 overexpression in the anterior lens capsule may contribute to the senescence of human lens epithelial cells in age-related cataract

Figure 8. Elevated TGF-β1 and laminin α4 (LMα4) in the cataractous anterior lens capsules (ALCs) with senescence. Human ALCs, graded before surgery for cataract severity by the Emery-Little Classification System of nuclear opacity grade, were obtained from the anterior surface of cataractous lenses during surgery. (A) Total TGF-β1 in human ALCs with ARC of grade II and grade V groups, as detected by ELISA. The figure depicts a Pearson correlation between TGF-β1 expression and senescence (n = 22) (B). (C) LMα4 subunit in human ALC groups groups with ARC grades II and V, as detected by ELISA. The figure depicts a Pearson correlation between LMα4 expression and senescence (n = 64) (D). (E) Association between LMα4 and total LM in ALCs with ARC (n = 60). (F) Association of LMα4 and TGF-β1 in ALCs with ARC (n = 9). Data were shown as mean ± SD and were analyzed using the Wilcoxon Rank Sum Test. *, p<0.05.