Research Paper Volume 11, Issue 9 pp 2762—2786

MiR-191 inhibit angiogenesis after acute ischemic stroke targeting VEZF1

Figure 5. miR-191 inhibited tube formation and spheroid sprouting. (A, B) Phase-contrast microscopic images of tubeforming HUVECs that were transfected with miR-191 mimic (A), miR-191 inhibitor (B), or the corresponding scrambled NCm (A) and NCi (B). Scale bars, 20μm. (C, D) Tube formation of HUVECs (percentage of NCm or NCi) transfected with miR-191 mimic (C) or miR-191 inhibitor (D) as assessed by tube formation assay (n =6 per group). (E, F) Phase-contrast microscopic images of sprouting HUVECs spheroids. HUVECs were transfected with miR-191 mimic (E), miR-191 inhibitor (F), or the corresponding scrambled NCm (E) and NCi (F). Scale bars, 20μm. (G, H) Sprouting coverage area of HUVECs (percentage of NCm or NCi) transfected with miR-191 mimic (G) or miR-191 inhibitor (H) as assessed by spheroid sprouting assay (n = 6 per group). Means ± SEM. ** P< 0.01 vs. NCm or NCi.