Figure 6. SIRT6 is a target of miR-138 and is inhibited by HMMR-AS1 knockdown. (A, B) Schematic view of miR-138 putative binding site in the WT/Mut 3′-UTR of SIRT6. Luciferase activity assay in HEK-293T cells transfected with luciferase report plasmids with WT/Mut SIRT6 3′-UTR or miR-138. (C, D) Relative mRNA and protein levels of SIRT6 in NCI-H23 and A549 cells after transfection. (E) SIRT6 mRNA and protein level in NCI-H23 and A549 cells after HMMR-AS1 knockdown. (F) SIRT6 mRNA and protein level in NCI-H23 cells after HMMR-AS1 knockdown and/or miR-138 inhibition. F. Correlation between HMMR-AS1 and SIRT6 expressions in 18 paired LUAD tissues. *P < 0.05, **P < 0.01.