Research Paper Volume 11, Issue 10 pp 3041—3054

LncRNA HMMR-AS1 promotes proliferation and metastasis of lung adenocarcinoma by regulating MiR-138/sirt6 axis

Figure 7. Effects of SIRT6 knockdown on viability of LUAD cells. (A) The SIRT6 protein expression was determined by western blot after SIRT6 knockdown. (B, C) NCI-H23 and A549 cell growth were determined by CCK8 assays and EdU staining assays. (D) Cell cycle was analyzed by flow cytometry. (E) The apoptotic rates of cells were detected by flow cytometry. (F, G) Cell ability of NCI-H23 and A549 cells co-transfection with si-SIRT6 and miR-138 inhibitor was detected by CCK8 and colony formation assays. (H, I) Cell ability of NCI-H23 and A549 cells co-transfection with si-SIRT6 and HMMR-AS1 was detected by CCK8 and colony formation assays. *P < 0.05, **P < 0.01.