Research Paper Volume 11, Issue 9 pp 2852—2873

Poly(ADP-ribosyl)ation and DNA repair synthesis in the extracts of naked mole rat, mouse, and human cells

Figure 3. Demonstration of primer MARylation in Mmu WCE (A) and in the system reconstituted from recombinant proteins (B). (A) Mmu cell extract proteins (0.5 mg/mL) were incubated for 5 min with 100 nM DNA duplexes bearing dRP, pDEG, or flap in the presence of 5 mM MgCl2 and 0.5 mM NAD+ in the absence or presence of PARG and olaparib. (B) Recombinant proteins were incubated for 10 min with 100 nM DNA duplexes bearing dRP, pDEG, or flap in the presence of 5 mM MgCl2, 0.1 mM dNTPs, and 0.5 mM NAD+ (when indicated). Lanes 1 in A and B correspond to the initial primer (control).