Research Paper Volume 11, Issue 10 pp 3055—3079

MiR-320a induces diabetic nephropathy via inhibiting MafB

Figure 5. Overexpression of miR-320a down-regulated MafB in vitro and in vivo. Relative MafB (A) mRNA and (B) protein levels in differently treated db/db mice. Relative Nephrin and Gpx3 (C) mRNA and (D) protein expression in differently treated db/db mice. Data are expressed as mean ± SEM, n=8, *P<0.05 versus C57BL/Ks, #P<0.05 versus db/db control, &P<0.05 versus db/db control. Relative MafB (E) mRNA and (F) protein levels in cultured podocyte cells. Relative Nephrin and Gpx3 expression measured by (G) real-time PCR and detected by (H) western blot. Data are expressed as mean ± SEM, n=3, *P<0.05 versus NG, #P<0.05 versus HG + miR-con, &P<0.05 versus HG + inhibitor-con.