Research Paper Volume 11, Issue 10 pp 3080—3093

The in vitro effects of gibberellin on human sperm motility

Figure 3. Gibberellin exposure reduced ATPase activity. (A) Na+/K+-ATPase and Ca2+-ATPase activity in sperm were detected with an ATP assay kit. (B–C) The protein levels of ATP-5A and ATP-5B in sperm were detected by immunocytochemistry and quantified based on the relative fluorescence intensity. (D–E) The relative protein levels of ATP-5A and ATP-5B in sperm were detected by Western blotting. The results are presented as the mean ± SEM.