Research Paper Volume 11, Issue 10 pp 3094—3116

Tyrosine nitrations impaired intracellular trafficking of FSHR to the cell surface and FSH-induced Akt-FoxO3a signaling in human granulosa cells

Figure 1. Molecular characteristics of human GCs from non-POR and POR groups. (A) The apoptotic indices of human GCs were measured using in Situ cell death detection kit. (B) The caspase-3 enzyme activities of human GCs were assayed using a commercial kit. (C) Total ROS levels of human GCs were measured by DCFH. (D) Relative mRNA expressions of FSHR in human GCs were determined by RT-PCR (β-actin as internal standard). (E) Relative protein expressions of FSHR in human GCs were determined by immunoblots (β-actin as internal standard). (F) PN levels of human GCs were measured by DHR. (G) The SOD activities of human GCs were measured using SOD activity assay kit. (H) Identification of tyrosine nitrated FSHR protein in human GCs. FSHR proteins were purified from cell lysates, and the relative protein expressions of nitrated FSHR were determined by immunoblots (β-actin as internal standard). Open triangle: p<0.05 vs. Non-POR group (n = 6–10).