Research Paper Volume 11, Issue 9 pp 2898—2910

miR-495 inhibits the growth of fibroblasts in hypertrophic scars

Figure 3. miR-495 inhibits HSF growth. (A) MTT assay showing that following transfection with miR-495 mimic/inhibitor, HSF proliferation is repressed/enhanced. Data are presented as the mean ± SEM. ** P< 0.01 vs. control. (B) AV-PI assay showing that following transfection with miR-495 mimic/inhibitor, HSF apoptosis is enhanced/repressed. (C, D) Following transfection of HSFs with miR-495 mimic, expression of FAK, FAKp-Tyr397, cyclin D1, bcl-2, bax and COL1A were detected using western blotting and real-time PCR. Data are presented as the mean ± SEM. ** P< 0.01. (E, F) Following downregulation of miR-495, expressions of FAK, FAKp-Tyr397, cyclin D1, bcl-2, bax and COL1A were detected using western blotting and real-time PCR. Data are presented as the mean ± SEM. ** P< 0.01.