Figure 10. Inflammation elevated in aging zebrafish retina and not regulated by resveratrol treatment (A) Representative western blot showing the protein expression level of iNOS in young, resveratrol-treated young, aging, and resveratrol-treated aging zebrafish retinas. (B) The densitometric mean and SEM of bands from A normalized to the corresponding level of the loading control protein beta-tubulin (**P<0.01, n=3). (C-E) Cox-2, TNF-α, and IL-1β expression in young, resveratrol-treated young, aging, and resveratrol-treated aging zebrafish retinas. Graphs represent (C) Cox-2, (D) TNF-α, and (E) IL-1β gene expression by quantitative real-time PCR (mean ± SEM, *P<0.05 **P<0.01, n=3). young, 4-6 month old zebrafish; young + RES, young zebrafish after resveratrol treatment for 10 day; aging, 19-23 month old zebrafish; aging + RES, aging zebrafish after resveratrol treatment for 10 days.