Figure 5. Resveratrol treatment increased mitophagy in young zebrafish retina. (A) Pink1 gene expression in adult zebrafish retina after treatment with resveratrol for 1 and 10 days as measured by quantitative real-time PCR (mean ± SEM, *P<0.05 compared to control (CON), n=3). (B) Representative western blot showing the protein expression of Pink1 in adult zebrafish retina after treatment with resveratrol for 1 and 10 days. (C) Graph of the densitometric mean and SEM normalized to the corresponding level of the loading control protein beta-tubulin from western blots as in B (*P<0.05 compared to control (CON), n=3). (D) Immunofluorescence localization and relative expression of Pink1 in the RGC layer of adult zebrafish retinas after treatment with resveratrol for 10 days. Retina cross sections prepared from zebrafish eyes were immunostained with Pink1 antibody. All photographs were taken at 40x magnification. CON, control; RES1d, resveratrol treated for 1 days; RES/RES10d, resveratrol treated for 10 days; GCL, ganglion cell layer.