Figure 5. The function of miR-34b in patients with uraemia. (A) Alizarin Red S staining showed normal renal arteries from healthy donors and calcified renal arteries from uraemia patients. n = 5. Representative images were shown. The arrows indicate the calcification sites of the human renal artery. (B) qRT-PCR detected the expression of miR-34b in arteries from healthy donors and uraemia patients, respectively. (C) The methylation rate of CpG sites of miR-34 DNA in arteries from uraemia patients was significantly higher than that from normal donors. n = 3. Representative images were shown. (D–F) The levels of DNMT3a and Notch1 were determined by western blot and immunohistochemistry staining analysis in arteries from healthy donors and uraemia patients. (G) The proposed model of miR-34b regulating the process of vascular calcification. The data were expressed as mean ± SD, *p < 0.05.