Figure 2. Simvastatin upregulated miR-140-5p expression. (A) Venn diagram containing miRNAs that were found to be significantly altered in triple negative tumors (ER-, PR- and Her2-) compared with other breast tumors (ER+ and/or PR+ and/or Her2+) and were regulated by statins. (B) KEGG pathway showed targeted genes of the 8 different miRNAs came from A. (C) qPCR analysis of the 8 different miRNAs expression in MDA-MB-231 cells treated with 3µM simvastatin compared with negative control DMSO for 24h. (D) The relative miRNA expression levels of miR-140-5p and miR-140-3p in MDA-MB-231 cells. (E, F) The expression levels of miR-140-5p and miR-140-3p were detected by qPCR in MDA-MB-231 cells treated with simvastatin(1-5µM) for 24h. All miRNAs expression was normalized to snRNA U6 housekeeping gene. The p-values were calculated using standard Student t-tests. Error bars represent mean±SEM of three individual experiments. *** P ≤ 0.001, ** P ≤ 0.01.