Figure 2. NRSF deficiency aggravates early damage in the nigrostriatal pathways induced by MPTP. (A) Immunohistochemical staining of TH in the substantia nigra at 7 days after saline or MPTP injection. Scale bar: 200 μm. Statistics for TH- and Nissl-positive neurons are shown in the right panel. N = 5-6. (B) Western blot analysis of TH expression in the striatum. Quantification of relative TH expression is shown in the right panel. N = 4. (C) Immunohistochemical staining of TH in the striatum. Scale bar: 200 μm. Densitometric analysis of TH staining is shown in the lower right panel. N = 4. All data are means ± SEM. Differences were analyzed by one-way ANOVA. ***p < 0.001, vs saline-treated control; #p < 0.05, ##p<0.01, ### p < 0.001, vs MPTP-treated WT mice.