Research Paper Volume 11, Issue 10 pp 3280—3297

Brain-specific NRSF deficiency aggravates dopaminergic neurodegeneration and impairs neurogenesis in the MPTP mouse model of Parkinson’s disease

Figure 3. NRSF deficiency potentiates late damage in the nigrostriatal pathways induced by MPTP. (A) Immunohistochemical staining of TH in the substantia nigra at 42 days after saline or MPTP injection. Scale bar: 200 μm. Statistics for TH- and Nissl-positive neurons are shown in the right panel. N = 4. (B) Western blot analysis of TH levels in the striatum. Quantification of relative TH expression is shown in the right panel. N = 4. (C) Immunohistochemical staining of TH in the striatum. Scale bar: 200 μm. Densitometric analysis of TH staining is shown in the lower right panel. N = 4. Data are means ± SEM. Data were analyzed by one-way ANOVA. **p < 0.01, ***p < 0.001, vs saline-treated control; #p < 0.05, ##p < 0.01, ###p < 0.001, vs MPTP-treated WT mice.