Research Paper Volume 11, Issue 10 pp 3280—3297

Brain-specific NRSF deficiency aggravates dopaminergic neurodegeneration and impairs neurogenesis in the MPTP mouse model of Parkinson’s disease

Figure 4. NRSF deficiency correlates with increased astrocyte activation in the nigrostriatal pathways after MPTP administration. (A) Western blot analysis of striatal GFAP at 7 days after saline or MPTP administration. Quantification of GFAP expression is shown in the right panel. N = 5. (B) Immunofluorescence staining of GFAP (green) and TH (red) in the substantia nigra at 7 days after saline or MPTP administration. Scale bar: 100 μm. Statistics for GFAP-positive cells are shown in the right panel. N = 4. (C) Western blot analysis of striatal GFAP at 42 days after saline or MPTP administration. Quantification of relative GFAP expression is shown in the right panel. N = 4. (D) Immunofluorescence staining of GFAP (green) and TH (red) in the substantia nigra at 42 days after saline or MPTP administration. Scale bar: 100 μm. Data are means ± SEM. Data were analyzed by one-way ANOVA. *P < 0.05, **p < 0.01, ***p < 0.001, vs saline-treated control; #p < 0.05, ###p < 0.001, vs MPTP-treated WT mice.