Research Paper Volume 11, Issue 10 pp 3298—3314

GDF10 inhibits proliferation and epithelial-mesenchymal transition in triple‐negative breast cancer via upregulation of Smad7

Figure 4. Overexpression of GDF10 inhibits proliferation of BT-20 cells. GDF10 expression at the mRNA (A) and protein (B) levels in BT-20 cells 72 h after transfection with NC or GDF10 for 72 h. **P < 0.01, compared with the NC group. (C) Cell proliferation assay results. BT-20 were transfected with NC or GDF10 and the CCK-8 assay was conducted at 0, 24, 48, and 72 h. **P < 0.01, compared with the NC group. (D) Ki67 immunofluorescence quantification in NC and GDF10-overexpressing BT-20 cells. **P < 0.01, compared with the NC group. (E) Apoptosis rates detected through Annexin V/PI double staining and flow cytometry in NC and GDF10-overexpressing BT-20 cells. **P < 0.01, compared with the NC group. (F) Cell invasion assay. BT-20 cells were transfected with NC or GDF10 for 72 h and Matrigel-coated transwell inserts used to quantify their invasive capacity. **P < 0.01, compared with the NC group.