Research Paper Volume 11, Issue 10 pp 3362—3375

Circular RNA hsa_circ_0001649 inhibits hepatocellular carcinoma progression via multiple miRNAs sponge

Figure 3. Circ-0001649 and SHPRH regulated migration of HCC cells. (A) Transwell assay showed that upregulation of circ-0001649 inhibited cell migration, which was reversed by sh-SHPRH. (B) Conversely, downregulation of circ-0001649 promoted cell migration, which was reversed by SHPRH. (C) The protein and mRNA levels of epithelial marker, mesenchymal marker and EMT-related transcriptional active factor in HepG2 cells upon transfection of circ-0001649 overexpression vector or co-transfection of circ-0001649 overexpression vector and sh-SHPRH. (D) The protein and mRNA levels of epithelial marker, mesenchymal marker and EMT-related transcriptional active factor in SMMC-7721 cells upon transfection of sh-circ-0001649 or co-transfection of sh-circ-0001649 and SHPRH overexpression vector. *P < 0.05 versus control group, #P < 0.05 versus circ-0001649 or sh-circ-0001649 group, data represent the mean ± SD.