Research Paper Volume 11, Issue 10 pp 3376—3391

MicroRNA-335/ID4 dysregulation predicts clinical outcome and facilitates leukemogenesis by activating PI3K/Akt signaling pathway in acute myeloid leukemia

Figure 1. MiR-335 overexpression negatively correlated with ID4 underexpression in AML. (A) MiR-335 expression in controls and AML patients. MiR-335 expression examined by RT-qPCR was significantly upregulated in AML patients. (B) MiR-335 methylation level in controls and AML patients. MiR-335 methylation examined by RQ-MSP in AML patients was similar to controls. (C) MiR-335 methylation level in controls and AML patients obtained by bioinformatics analysis. MiR-335 promoter (CpG island) methylation level was obtained through the human disease methylation database DiseaseMeth version 2.0 (http://www.bio-bigdata.com/diseasemeth/analyze.html). (D) MiR-335 methylation density in controls and representative AML patients. MiR-335 methylation density was determined by BSP. Control 1 and Control 2 indicated two controls were similar to AML 1 and AML 2 represented two AML patients. (E) ID4 expression level in controls and AML patients. ID4 expression examined by RT-qPCR was significantly down-regulated in AML patients. (F) Correlation between miR-335 and ID4 expression in AML. A negative correlation was observed between miR-335 and ID4 expression. The correlation analysis was conducted by Spearman test.