Research Paper Volume 11, Issue 10 pp 3376—3391

MicroRNA-335/ID4 dysregulation predicts clinical outcome and facilitates leukemogenesis by activating PI3K/Akt signaling pathway in acute myeloid leukemia

Figure 3. MiR-335 exhibited pro-proliferative and anti-apoptotic effects in leukemic cell-lines. (A–B) Confirmation of miR-335 expression after miR-335 transfection. MiR-335 expression was significantly upregulated after miR-335 transfection in both K562 and HL60 cell-lines. (C–D) The effect of miR-335 overexpression on cell proliferation. Overexpression of miR-335 significantly increased the proliferation ability in K562 and HL60 cell-lines. (E–F) The effect of miR-335 overexpression on cell apoptosis. Overexpression of miR-335 significantly decreased the apoptosis ratio in K562 and HL60 cell-lines. (G) The expression of proliferation-related proteins (PCNA and Cyclin D1) and apoptosis-related proteins (Caspase-3) affected by miR-335 overexpression. The expression of proliferation-related proteins (PCNA and Cyclin D1) was increased, whereas the apoptosis-related proteins (Caspase-3) expression was decreased after miR-335 overexpression in K562 and HL60 cell-lines. *, P<0.05; **, P<0.01; ***, P<0.001.