Research Paper Volume 11, Issue 11 pp 3716—3730

Long non-coding RNA MEG3 promotes fibrosis and inflammatory response in diabetic nephropathy via miR-181a/Egr-1/TLR4 axis

Figure 3. Effect of MEG3 on the secretion of inflammatory cytokines in vitro and in vivo. (AD) ELISA analysis results showed that the concentrations of CRP (A), IL-1β (B), IL-6 (C), and MCP-1 (D) in the supernatant of MCs were upregulated. MCs were transfected with LV-MEG3; (EH) ELISA analysis results showed that the concentrations of CRP (E), IL-1β (F), IL-6 (G), and MCP-1 (H) in the supernatant of MCs were downregulated. MCs were transfected with siRNA-MEG3; (IL) The concentrations of CRP (I), IL-1β (J), IL-6 (K), and MCP-1(L) in the kidney cortex of DN rats were upregulated. DN rats were injected with LV-MEG3.