Figure 3. Validation results of the candidate senescence bypass genes. (A) The senescence bypass caused by different candidate genes knockout was detected with SA-β-gal staining. Representative images were shown. (B) Representative images of senescence bypass cells stained by the proliferative marker Ki67. (C) Percentage of β-gal positive cells at 27 days after bleomycin induction (n = 3, mean ± SD). *p < 0.05; **p < 0.01; ***p < 0.001 by t-test in comparison with control cells. (D) Percentage of Ki67 positive cells at 27 days after bleomycin induction in samples knocking-out candidate genes (n = 3, mean ± SD).