Research Paper Volume 11, Issue 12 pp 4011—4031

A large-scale CRISPR screen and identification of essential genes in cellular senescence bypass

Figure 3. Validation results of the candidate senescence bypass genes. (A) The senescence bypass caused by different candidate genes knockout was detected with SA-β-gal staining. Representative images were shown. (B) Representative images of senescence bypass cells stained by the proliferative marker Ki67. (C) Percentage of β-gal positive cells at 27 days after bleomycin induction (n = 3, mean ± SD). *p < 0.05; **p < 0.01; ***p < 0.001 by t-test in comparison with control cells. (D) Percentage of Ki67 positive cells at 27 days after bleomycin induction in samples knocking-out candidate genes (n = 3, mean ± SD).