Research Paper Volume 11, Issue 12 pp 4050—4065

TRPM7 channel inhibition exacerbates pulmonary arterial hypertension through MEK/ERK pathway

Figure 1. TRPM7 expression is downregulated in PASMCs from PAH human and a rat model. (A) The mRNA level of TRPM7 in PASMCs from control (n = 6) or PAH (n = 8) human donors was determined by qRT-PCR analysis. β-actin was used as a reference control. Data are mean ± SD. Unpaired Student’s t-test. **, P < 0.01 compared to control. (B) The protein level of TRPM7 in PASMCs from 3 representative control and PAH human donors was determined by Western blot analysis. β-actin was used as a loading control. The representative images (left) and band intensity analysis (right) are shown. Data are mean ± SD. Unpaired Student’s t-test. **, P < 0.01 compared to control. (C) The mRNA level of TRPM7 in PASMCs from control (n = 5) or hypoxia-induced PAH (n = 5) rats was determined by qRT-PCR analysis. β-actin was used as a reference control. Data are mean ± SD. Unpaired Student’s t-test. **, P < 0.01 compared to control. (D) The protein level of TRPM7 in PASMCs from 3 representative control and hypoxia-induced PAH rats was determined by Western blot analysis. β-actin was used as a loading control. The representative images (left) and band intensity analysis (right) are shown. Data are mean ± SD. Unpaired Student’s t-test. **, P < 0.01 compared to control.