Research Paper Volume 11, Issue 12 pp 4050—4065

TRPM7 channel inhibition exacerbates pulmonary arterial hypertension through MEK/ERK pathway

Figure 5. TRPM7 overexpression reverses proliferation and apoptosis resistance in PASMCs. (A) PASMCs from rats were infected with adenovirus expressing control vector (Ad-Ctrl) or TRPM7 (Ad-TRPM7). After 72 h of infection, the protein level of TRPM7 was determined by Western blot analysis. β-actin was used as a loading control. The representative images from 3 independent experiments are shown. (BD) PASMCs from rats were infected with Ad-Ctrl or Ad-TRPM7. After 24 h of infection, cells were serum starved for 24 h, followed by incubation with medium containing 5% FCS for another 24 h. (B) Cell proliferation was determined by BrdU incorporation assay. (C) Cell apoptosis was detected by TUNEL staining. Results are expressed as a percentage relative to control. Data are mean ± SD. n = 3. One-way ANOVA test. **, P < 0.01 compared to vector. (D) The protein expression of cleaved caspase-3 was determined by Western blot analysis. β-actin was used as a loading control. The representative images from 3 independent experiments are shown.