Figure 3. Murine ISCs show increased DNA DSB and cell proliferation after heavy ion radiation exposure. (A) Representative immunofluorescence (IF) images of murine ISC sections from control and irradiated mice showing increased γH2AX foci in nuclei after iron irradiation. Scale bars, 5 μm. (B) Number of γH2AX foci in ISC nuclei from control and irradiated mice were counted and data from five mice presented graphically showing significantly increased foci in irradiated samples. (C) Representative IF images showing 53BP1 foci in ISC sections from control and irradiated samples. Scale bars, 5 μm. (D) Quantification of 53BP1 foci in control and irradiated sections presented graphically showing increased number of foci in iron irradiated samples. (E) Representative IF images of ISC sections stained for PCNA showing increased staining in irradiated relative to control samples. Scale bars, 10 μm. (F) Graphical presentation of percent of PCNA positive nuclei in control and irradiated samples from five mice. Error bars show SEM.