Research Paper Volume 11, Issue 12 pp 4183—4197

Alpha-ketoglutarate extends Drosophila lifespan by inhibiting mTOR and activating AMPK

Figure 2. Effects of AKG on reproduction, vertical climbing ability, and stress tolerance in wDah flies. (A) Fecundity assay. AKG decreased fecundity at concentrations of 1 and 5 μM (1 μM vs 0 μM AKG, p = 0.0013; 5 μM vs 0 μM AKG, p = 0.005; Wilcoxon rank test) and had no significant effect at 10 μM (10 μM vs 0 μM AKG, p > 0.05, Wilcoxon rank test). (B) RING assay. AKG-supplementation increased vertical climbing ability at 3 time points (20, 30, and 40 days) compared with the control diet (*p < 0.05 and **p < 0.01, two-tailed Student’s t-test). (C) Heat stress assay. AKG-treated flies were significantly more resistant to 39°C heat stress than control flies (5 μM AKG vs control, p = 0.0023, log-rank test). (D) Paraquat stress assay. There were no differences in sensitivity to paraquat stress between control and AKG-treated flies (5 μM AKG vs control, p > 0.05, log-rank test). (E) H2O2 stress assay. There were no differences in sensitivity to H2O2 stress between control and AKG-treated flies (5 μM AKG vs control, p > 0.05, log-rank test). All data are shown as the mean ± standard error of the mean (SEM).