Research Paper Volume 11, Issue 14 pp 4990—5007

Long noncoding RNA FALEC inhibits proliferation and metastasis of tongue squamous cell carcinoma by epigenetically silencing ECM1 through EZH2

Figure 2. FALEC silence promotes the proliferation and migration of TSCC cells. (A) RT-qPCR analysis of FALEC was determined in FALEC-silenced and control SCC-4 and SCC-25 cell lines. (B) CCK-8 assays were used to determine the viability of FALEC-silenced TSCC cell lines. (C) EdU assays were performed to determine the proliferation of TSCC cell lines when FALEC was knockdown. (D) Colony formation assays were used to determine the colony-forming ability of FALEC-silenced cells. Representative images (left) and average number of colonies (right) are shown. (E) Transwell assays showed that FALEC silencing promoted TSCC cells migration. Representative images (left) and average number of cells (right) are shown. Data are shown as means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.