Research Paper Volume 11, Issue 14 pp 4990—5007

Long noncoding RNA FALEC inhibits proliferation and metastasis of tongue squamous cell carcinoma by epigenetically silencing ECM1 through EZH2

Figure 5. ECM1 is highly expressed in TSCC and negatively controlled by FALEC. (A) Human FALEC locus was shown using UCSC Genome Browser. There is a divergent mRNA in near from FALEC locus (B) RT-qPCR analysis of FALEC in the subcellular fractions of SCC-25 and SCC-4 cells. U6 and GAPDH acted as nuclear and cytoplasmic markers, respectively (n=3). (C) Correlation of FALEC expression and ECM1 expression in primary TSCC samples and normal samples. (D) RT-qPCR analysis of ECM1 in FALEC overexpressing or knockdown TSCC cell lines. (E) Western blotting of ECM1 in FALEC overexpressing or knockdown TSCC cell lines. (F) Representative immunostaining of ECM1 in FALEC overexpressing and control xenografted tumors. (G) RT-qPCR analysis of ECM1 in FALEC overexpressing and control xenografted tumors. (H) Representative immunostaining of ECM1 in TSCC tissues and adjacent normal tissues. (I) RT-qPCR analysis of ECM1 in TSCC tissues and adjacent normal tissues. Data are shown as means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.