Figure 7. FALEC Binds with EZH2 to Epigenetically Silence ECM1, Inhibiting Cell Proliferation and Migration in TSCC Cell Lines. (A) Western Blotting of proteins from antisense FALEC and sense FALEC pull-down assays (n=3). (B) RNA immunoprecipitation (RIP) experiments were performed using the EZH2 antibody, and specific primers were used to detect FALEC (n=3). (C) ECM1 level was detected by RT-qPCR in the TSCC cell lines after overexpression or knockdown of EZH2. (D) ECM1 protein level was detected by western blotting in the TSCC cell lines after overexpression or knockdown of EZH2. (E, F) chromatin immunoprecipitation (ChIP) assays of EZH2 and H3K27me3 of the promoter region of the ECM1 locus after FALEC overexpression or knockdown. qPCR was performed to quantify the ChIP assays products. Enrichment was quantified relative to the input controls. IgG antibodies were used as a negative control. Data are shown as means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.