Research Paper Volume 11, Issue 14 pp 5008—5034

Ginsenoside Rb1 regulates prefrontal cortical GABAergic transmission in MPTP-treated mice

Figure 6. Rb1 promotes GABAARα1 receptor expression in the PFC in MPTP-treated mice. (A and B) The effect of Rb1 on total GABAARα1 expression and expression in the membrane fraction of the PFC of MPTP-treated mice was determined by western blotting. (C) The effect of Rb1 on GAD67 and gephyrin expression in the PFC of MPTP-treated mice was determined by western blotting. (D) Immunofluorescence staining of GABAARα1 (green), gephyrin (red) and NeuN (purple) in the PFC of MPTP-treated mice. Nuclei are labeled with DAPI (blue). Scale bar = 10 μm. Western blotting results are from two of the six mice in each group and are expressed as the mean ± SEM of three experiments. **P < 0.01 and *P < 0.05 vs. control; ##P < 0.01 and #P < 0.05 vs. MPTP group. Statistical significance was determined by one-way ANOVA and the Bonferroni post-hoc test for pairwise comparisons.