Research Paper Volume 11, Issue 14 pp 5035—5057

Overexpression of KLF5 is associated with poor survival and G1/S progression in pancreatic cancer

Figure 8. Western blot analysis confirming the regulatory relationships between KLF5 and its target genes (A) KLF5 expression in pancreatic cancer cell lines and a normal pancreatic cell line. (B) The density of each band was measured and normalized to the β-actin band. * P <0.05, ** P <0.01 vs. the HPDE6C7 group. (C) KLF5 expression after transfection of cell with KLF5 siRNA. (D) The density of each band was measured and normalized to the β-actin band. * P <0.05, ** P <0.01 vs. the negative control (NC) group. (E) PANC-1 cells were transfected for 48 h with empty vector or KLF5-EGFP plasmid, after which the protein extracts were assayed by western blotting. β-actin was used as a protein loading control. (F) BxPC-3 cells were transfected for 48 h with negative control siRNA, siKLF5-2 or siKLF5-3, after which the protein extracts were assayed by western blotting. β-actin was used as a protein loading control. (G) The density of each band was measured and normalized to the β-actin band. * P <0.05, ** P <0.01 vs. the empty vector group. (H) The density of each band was measured and normalized to the β-actin band. * P <0.05, ** P <0.01 vs. the NC group.