Research Paper Volume 11, Issue 14 pp 5058—5069

miR-548d-3p inhibits osteosarcoma by downregulating KRAS

Figure 2. MiR-548d-3p inhibits KRAS in osteosarcoma cells. (A) Correlation analysis between miR-548d-3p and KRAS expression. The correlation formula was y=-0.4602x+0.9542. (B) A luciferase reporter gene assay confirmed that miR-548d-3p could bind to KRAS. The results represent the mean±SD of three independent experiments. **P < 0.05 vs. control. (C–J) The protein and mRNA levels of KRAS were measured in MG63 and U2-OS cells by Western blotting and real-time PCR, respectively. The results represent the mean±SD of three independent experiments. **P < 0.05 vs. control.