Research Paper Volume 11, Issue 14 pp 5058—5069

miR-548d-3p inhibits osteosarcoma by downregulating KRAS

Figure 4. MiR-548d-3p inhibits the growth and migration of osteosarcoma cells by downregulating KRAS. (A) After transfection with KRAS overexpression vectors, cells were transfected with miR-548d-3p mimics or control miRNA. Cell growth was measured with an MTT assay after 24 h of transfection. The results represent the mean±SD of three independent experiments. **P < 0.05 vs. control, ##P < 0.05 vs. KRAS overexpression group. (B) After transfection with si-KRAS, cells were transfected with miR-548d-3p inhibitors or control miRNA. Cell growth was measured by an MTT assay after 24 h of transfection. The results represent the mean±SD of three independent experiments. **P < 0.05 vs. control, ##P < 0.05 vs. KRAS group or si-KRAS group. (CF) Cell migration was measured with a Transwell assay after 24 h of transfection. The results represent the mean±SD of three independent experiments. **P < 0.05 vs. control, ##P < 0.05 vs. KRAS group or si-KRAS group. (GN) The protein and mRNA levels of KRAS were measured in cells by Western blotting and real-time PCR, respectively. The results represent the mean±SD of three independent experiments. **P < 0.05 vs. control, ##P < 0.05 vs. KRAS group or si-KRAS group.