Research Paper Volume 11, Issue 19 pp 8068—8084

A circular RNA from APC inhibits the proliferation of diffuse large B-cell lymphoma by inactivating Wnt/β-catenin signaling via interacting with TET1 and miR-888

Figure 1. Identification of circ-APC as a DLBCL-related circRNA. (A) Heat map of the top five differentially expressed circRNAs in three paired DLBCL and normal tissues. (B and C) qRT-PCR analysis of circ-APC expression in DLBCL tissues and cells. (D) qRT-PCR analysis of circ-APC and APC mRNA expression after treatment with actinomycin D for the indicated times. (E) qRT-PCR analysis of circ-APC and APC mRNA expression after treatment with RNase R. (F) Schematic diagram of the origin of circ-APC. (G) RT-PCR analysis of circ-APC expression with convergent and divergent primers for cDNA or gDNA. (H) qRT-PCR analysis of cytoplasmic and nuclear circ-APC expression. *p < 0.05, **p < 0.01, ***p < 0.001.