Research Paper Volume 11, Issue 19 pp 8068—8084

A circular RNA from APC inhibits the proliferation of diffuse large B-cell lymphoma by inactivating Wnt/β-catenin signaling via interacting with TET1 and miR-888

Figure 2. Exogenous expression of circ-APC inhibits DLBCL cell proliferation both in vitro and in vivo. (A) qRT-PCR analysis of circ-APC expression in U2932 and TMD8 cells infected with a circ-APC-overexpressing lentiviral vector. (B) CCK-8 assay for DLBCL cell viability at the indicated times. (C) EdU assay to determine the DNA synthesis rate in control or stably circ-APC-overexpressing U2932 and TMD8 cells. Nuclei were stained by DAPI. (D) Cell cycle analysis (G0/G1, S, G2/M phase) in control or stably circ-APC-overexpressing U2932 and TMD8 cells. (E) The volumes and weights of the subcutaneous tumors in the control and circ-APC-overexpressing groups (n=6 per group). *p < 0.05, **p < 0.01, ***p < 0.001.