Research Paper Volume 11, Issue 19 pp 8068—8084

A circular RNA from APC inhibits the proliferation of diffuse large B-cell lymphoma by inactivating Wnt/β-catenin signaling via interacting with TET1 and miR-888

Figure 5. Circ-APC recruits TET1 to the APC promoter to enhance the transcription of APC in DLBCL. (A) qRT-PCR analysis of APC expression in U2932 and TMD8 cells treated with the indicated concentrations of 5-Aza-dC. (B) qRT-PCR analysis of APC expression in U2932 and TMD8 cells transfected with a TET1, TET2 or TET3 overexpression vector. (C) RPISeq online tool predicting the probability of binding between circ-APC and TET1. (D) RNA pull-down assay with a control or circ-APC probe, followed by Western blot analysis of TET1 expression. (E) RNA immunoprecipitation assay with an IgG or TET1 antibody, followed by qRT-PCR analysis of circ-APC expression. (F) ChIRP assay with a control or circ-APC probe, followed by qRT-PCR analysis of the APC promoter region. (G and H) ChIP assay with an IgG, TET1 or 5hmC antibody, followed by qRT-PCR analysis of the APC promoter region in U2932 and TMD8 cells after the indicated transfection. (I) qRT-PCR analysis of APC expression in stably circ-APC-overexpressing U2932 and TMD8 cells transfected with si-TET1. *p < 0.05, **p < 0.01, ***p < 0.001.