Research Paper Volume 11, Issue 16 pp 5992—6013

LncRNA RP11-670E13.6, interacted with hnRNPH, delays cellular senescence by sponging microRNA-663a in UVB damaged dermal fibroblasts

Figure 6. hnRNPH directly bound to RP11-670E13.6 and repressed its expression. (A) Pull down results of RP11-670E13.6 by silver staining (a) and western blot analysis (b) demonstrated the possible interactions between RP11-670E13.6 and hnRNPF/H. (B) RIP assays demonstrating the enrichment of hnRNPF/H on RP11-670E13.6 transcripts relative to IgG in HDFs. (C) Knockdown of both hnRNPH and hnRNPF had no effect on the expression of RP11-670E13.6. (D) Effects of HNRNPH1 siRNA on the expression of RP11-670E13.6 and hnRNPF. (E) Effects of HNRNPF siRNA on the expression of RP11-670E13.6 and hnRNPH. (F) The mRNA expression levels of HNRNPH1. (G) HnRNPH/F expression levels of HDFs treated with RP11-670E13.6 siRNA and UVB irradiation. (H) The mRNA expression levels of HNRNPH1. (I) hnRNP H/F expression levels of HDFs treated with UVB irradiation(40mJ/cm2). (J) CCK-8 assays were used to detect the effects of HNRNPH1 on HDFs viability. Data are shown as the means ± standard errors of the means based on at least three independent experiments. P values were determined by Student’s t-tests. *P < 0.05; **P < 0.01; and ***P < 0.001.