Research Paper Volume 11, Issue 16 pp 6053—6068

LINC01198 promotes proliferation and temozolomide resistance in a NEDD4-1-dependent manner, repressing PTEN expression in glioma

Figure 3. LINC01198 functions as a scaffold for NEDD4-1/PTEN to regulate PTEN expression in glioma cells. (A) Predicted interaction probabilities of LINC01198 and RNA-binding proteins via a RNA–protein interaction prediction tool. (B) RIP assays of LINC01198 binding to indicated proteins in glioma U87 cell extracts. (C) RNA pulldown was used to examine the association of LINC01198 and NEDD4-1/PTEN. (D) The LINC01198 gene was cut into LINC01198-D1(1-500 nt) and -D2(501-955 nt), and their overexpression vectors were constructed and transfected into U87 cells. (E) RIP assays of LINC01198-D1/D2 binding to indicated proteins in glioma U87 cell extracts. (F) Biotinylated LINC01198-D1/D2 RNAs were incubated with U873 cell lysates, and western blotting analysis was performed to evaluate the specific association between them and NEDD4-1/PTEN.