Research Paper Volume 11, Issue 16 pp 6053—6068

LINC01198 promotes proliferation and temozolomide resistance in a NEDD4-1-dependent manner, repressing PTEN expression in glioma

Figure 4. LINC01198 increased NEDD4-1-induced PTEN inhibition in glioma cells. (A) Forced LINC01198 expression inhibited PTEN expression in glioma cells. (B) Protein expression levels of PTEN were increased in glioma cells treated with LINC01198 shRNA. (C) Forced LINC01198 expression did not affect NEDD4-1 expression in glioma cells. (D) Protein expression levels of NEDD4-1 did not change in glioma cells treated with LINC01198 shRNA. (E) Forced LINC01198 expression did not inhibit PTEN expression in CRISPR/cas9-induced NEDD4-1-knockout glioma cells. (F) Protein expression levels of PTEN were not increased in CRISPR/cas9-induced NEDD4-1-knockout glioma cells treated with LINC01198 shRNA. (G and H). A negative correlation between LINC01198 and PTEN was observed in tumor tissues at the protein level (r = 0.501; P = 0.013), but this correlation was not observed at the mRNA level (r = 0.296; P > 0.05).