Research Paper Volume 11, Issue 16 pp 6273—6285

Elevated kindlin-2 promotes tumour progression and angiogenesis through the mTOR/VEGFA pathway in melanoma

Figure 2. High levels of kindlin-2 induce the cellular EMT in melanoma. (A) The cell morphology of SK28-shNC, SK28-sh-kindlin-2, A375-Vector, and A375-kindlin-2 cells was observed by a phase-contrast microscopy. (B) Correlation analyses between the expression of kindlin-2 and N-cadherin, β-catenin, ZEB1, ZEB2 were performed with the TCGA database. (C and E) The mRNA levels of N-cadherin, β-catenin, ZEB1 and ZEB2 in SK28-shNC, SK28-sh-kindlin-2, A375-Vector and A375-kindlin-2 cells were analyzed by qRT-PCR. (D and F) The protein levels of N-cadherin, β-catenin, ZEB1 and ZEB2 in the indicated cells were analyzed by western blotting. *p<0.05, **p<0.01, ***p<0.001.