Research Paper Volume 11, Issue 16 pp 6336—6357

Inhibition of de novo ceramide biosynthesis affects aging phenotype in an in vitro model of neuronal senescence

Figure 5. Effects of aging and L-CS on senescence-related molecular markers.Western blots show L-CS-or vehicle-driven effects on senescence-associated markers obtained from protein extracts of control and aged cortical cultures. Each image is representative of three independent experiments. (A) Bar graphs depict p53 levels in the four study groups (n=3). (B) Bar graphs depict pJNK levels in the four study groups (n=3). (C) Bar graphs depict pP38 levels in the four study groups (n=3). (D) Bar graphs depict pAKT levels in the four study groups (n=3). (E) Bar graphs depict pERK5 levels in the four study groups (n=3). Means were compared by two-way ANOVA followed by Tukey post-hoc test. * indicates p<0.05, ** indicates p<0.01, n.s. indicates not significant.