Research Paper Volume 11, Issue 16 pp 6371—6384

MiR-27a promotes the autophagy and apoptosis of IL-1β treated-articular chondrocytes in osteoarthritis through PI3K/AKT/mTOR signaling

Figure 5. Downregulation of miR-27a expression repressed the autophagy in IL-1β-triggered chondrocytes. (A) Twenty-four well plates were used to culture IL-1β-supplemented chondrocytes. The cells were first co-transfected for 36 h with the GFP-LC3B construct, as well as the miR-27a inhibitor/miR-Scr. The cellular location of GFP-LC3B was then evaluated via IFA (magnification, ×400). (B) WB was used to analyze the expression levels of LC3B and p62 in IL-1β-supplemented chondrocytes transfected with the miR-27a inhibitor. (C) Q-PCR was used to analyze the mRNA expression of p62 in IL-1β-treated chondrocytes transfected with the miR-27a inhibitor. The results were described as the mean ± SD. *P < 0.05, **P < 0.01 vs. indicated group.