Research Paper Volume 11, Issue 16 pp 6371—6384

MiR-27a promotes the autophagy and apoptosis of IL-1β treated-articular chondrocytes in osteoarthritis through PI3K/AKT/mTOR signaling

Figure 7. Silencing the PI3K expression restored the impact of miR-27a inhibition on the growth of IL-1β-treated chondrocytes. IL-1β-treated chondrocytes were co-transfected with the miR-27a inhibitor/miR-Scr and shRNA-PI3K/shRNA-NC. (A) The PI3K expression in cells after their transfection was assessed at the mRNA level by Q-PCR. (B) WB was performed to detect the Akt, phosphor-Akt, and mTOR protein levels in IL-1β-treated chondrocytes. (C) The proliferation rate of IL-1β-treated chondrocytes was examined at 24, 48, and 72 h following the transfection, utilizing the MTT test. **P < 0.01 vs. indicated group.