Figure 2. Age-related expression of Cav1.3 in auditory pathway. (A) The immunofluorescence of CaV1.3 in the auditory cortex (green, magnification, ×400). (B) the quantitative analysis of CaV1.3 expression in the auditory cortex. (C) the western-blotting analysis of CaV1.3 expression in auditory cortex (top), the bottom panel is the quantitative analysis. (D) the mRNA expression of CaV1.3 in auditory cortex, inferior colliculus and cochlear nucleus. (E) CaV1.3 expression in auditory cortex was analyzed by flow cytometry, the right panel is the quantitative analysis. (F) Cav1.3 expression in neurons of auditory cortex, the neuron cells were gated as NeuN+, the right panel is the quantitative analysis. Error bars represent mean ± s.d.; *P<0.05; **P < 0.01; ***P < 0.001; n.s. not significant; by one-way analysis of variance (ANOVA) (B–F).