Research Paper Volume 11, Issue 17 pp 7274—7293

The activation of cardiac dSir2-related pathways mediates physical exercise resistance to heart aging in old Drosophila

Figure 1. Influence of cardiac dSir2 knockdown on heart lipid accumulation and oxidative stress. (A) Cardiac dSir2 expression level. (B) Cardiac Foxo expression. (C) Cardiac SOD activity level. (D) Cardiac MDA level. (E) Cardiac bmm expression. (F) Cardiac TAG level. (G) The normal line of TAG, the normal line of SOD, and the normal line of MDA. Independent-sample t tests were used to assess differences between the 1-week-old and 7-week-old flies to explore the effects of aging on the heart. Independent-sample t tests were used to assess differences between hand-Gal4>w11118 and hand-Gal4>UAS-dSir2-RNAi flies to explore the effects of cardiac dSir2 knockdown on the heart. Data are represented as means ± SEM. *P<0.05; **P <0.01. Sample size was 80 hearts for each group, and measurements were taken 3 times.